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Two-dimensional monitoring of spiking networks in acute brain slices.
Egert U, Heck D, Aertsen A.
and Biophysics, Institute of Biology III, Albert-Ludwigs Universitat
Freiburg, Schanzlestr. 1, 79104 Freiburg, Germany.
To understand spatiotemporally
coordinated activity in neural networks and interaction between
different areas or layers in brain tissue, simultaneous multisite
recording is a prerequisite. For in vitro studies pursuing these goals,
substrate integrated, planar microelectrode arrays (MEAs) have been
developed to monitor spikes and local field potentials. Here we report
for the first time recordings of single-unit spike activity with MEAs
in acute slice preparations of the rat cerebellum. We compare these
recordings to results of conventional techniques, and discuss the
recording conditions in view of the equivalent circuits commonly used.
Simultaneous recordings with tungsten microelectrodes and MEAs verified
that recording characteristics and signal-to-noise ratios of MEA
electrodes were comparable to those of conventional extracellular
electrodes. Spike shapes were identical on both electrodes. We found no
detectable overlap between spike signals recorded at neighboring MEA
electrodes (200 microm spacing). Neuronal spike activity was detected
with MEA electrodes at distances of up to 100 microm from the site of
spike generation. We conclude that extracellular recording of
independent single-unit spike activity with MEAs is indeed suitable to
monitor network activity in acute slices, making MEAs an exceptionally
useful tool for the assessment of fast network dynamics in acute slices.
PMID: 11807580 [PubMed - indexed for MEDLINE]