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Voltage signals of individual Purkinje cell dendrites in rat cerebellar slices.
Borst A, Heck D, Thomann M.
Friedrich-Miescher-Laboratory of the Max Planck Society, Tuebingen, Germany. alexander.borst@tuebingen.mpg.de
For
investigating neuronal information processing at the cellular level, a
technique which visualizes the voltage distribution within single
neurons in situ would be extremely useful. Voltage-sensitive dyes are,
in principle, capable of reporting membrane potential [Cohen, L.B. and
Salzberg, B.M., Rev. Physiol. Biochem. Pharmacol., 83 (1978) 35-88;
Grinvald, A., Lieke, E.E., Frostig, R.D. and Hildesheim, R., J.
Neurosci., 14 (1994) 2545-2568; Kleinfeld, D., Delaney, K.R., Fee,
M.S., Flores, J.A., Tank, D.W. and Gelperin, A., J. Neurophysiol., 72
(1994) 1402-1419]. However, their application to single cells
internally is technically difficult [Antic, S. and Zecevic, D., J.
Neurosci., 15 (1995) 1392-1405; Grinvald, A., Salzberg, B.M., Lev-Ram,
V. and Hildesheim, R., Biophys. J., 51 (1987) 643-651; Kogan, A., Ross,
W.N., Zecevic, D. and Lasser-Ross, N., Brain Res., 700 (1995) 235-239;
Zecevic, D., Nature, 381 (1996) 322-325]. An alternative strategy
consists in applying the dye from the outside to all cells in the
tissue, while manipulating a single cell by current injection
[Krauthamer, V. and Ross, W.N., J. Neurosci., 4 (1984) 673-682; Ross,
W.N. and Krauthamer, V., J. Neurosci., 4 (1984) 659-672]. Here, we
modify this technique to further enhance spatial at the cost of
temporal resolution [Borst, A., Z. Naturforsch., 50 (1995) 435-438].
Applied to rat cerebellar slices we demonstrate that the potential
spread in individual Purkinje cells can be imaged up to even fine
dendritic branches. The acquired optical signals suggest that steadily
hyperpolarized Purkinje cells are electrically compact. When
permanently depolarized, the somatic input resistance is significantly
diminished, yet the spatial voltage drop along the dendrites remains
unchanged. As demonstrated by compartmental modeling, this hints to a
concentration of outward rectifying currents at the soma of the cells.
PMID: 9464647 [PubMed - indexed for MEDLINE]
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