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Current source density profiles of optical
recording maps: a new approach to the analysis of spatio-temporal
neural activity patterns.
Plenz D, Aertsen A.
Max-Planck-Institut fur biologische Kybernetik, Tubingen, Germany.
spreading of activity in the CA1 region of the rat hippocampal slice
was studied by two experimental approaches. At identical locations in
the tissue we measured both the extracellular field potential
distribution with microelectrode recordings and the intracellular
potential distribution by optical recording, using voltage-sensitive
fluorescent dyes. Current source density analysis (CSD) was applied to
the extracellular field potential distributions (eCSD) to enhance the
spatial resolution. In order to obtain an analogous improvement for the
optical recordings, we developed a new CSD transformation, which
calculates the locations of the transmembrane current generators from
the intracellular potential distributions (iCSD). Compared to the
underlying fluorescence maps, the new iCSD profiles exhibit a
considerable improvement in spatial resolution. Results can be directly
interpreted in terms of physiological membrane processes, such as
postsynaptic potentials and action potentials. The iCSD profiles show a
surprisingly good correspondence with the classical eCSD profiles both
qualitatively and quantitatively, the only difference being that cell
body activity is reduced in amplitude. Thus, this new optical CSD
analysis paves the way for a quantitative interpretation, rather than
the hitherto predominantly qualitative interpretation of
spatio-temporal activity profiles from optical recording measurements.
PMID: 8261120 [PubMed - indexed for MEDLINE]
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